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Mouse Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A) ELISA Kit

Mouse Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A) ELISA Kit

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Specification

Product name Mouse Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP1LC3A in samples. An antibody specific for MAP1LC3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAP1LC3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP1LC3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP1LC3A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MAP1LC3A; RP11-346K17.1; LC3; LC3A; MAP1ALC3; MAP1BLC3; MAP1 light chain 3-like protein 1; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; OTTHUMP00000030698; microtubule-associated proteins 1A/1B light chain 3

Product Properties

Kit components • Mouse Microtubule-associated proteins 1A/1B light chain 3A microplate
• Mouse Microtubule-associated proteins 1A/1B light chain 3A standard
• Mouse Microtubule-associated proteins 1A/1B light chain 3A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MAP1LC3A. No significant cross-reactivity or interference between Mouse MAP1LC3A and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.The deduced 121-amino acid protein shares 81% identity with rat Map1lc3. Northern blot analysis detected a 1.1-kb transcript in most tissues examined, with highest expression in heart, brain, liver, skeletal muscle, and testis. No MAP1LC2A expression was detected in thymus and peripheral blood leukocytes.
Gene ID 66734
Alternative MAP1LC3A; RP11-346K17.1; LC3; LC3A; MAP1ALC3; MAP1BLC3; MAP1 light chain 3-like protein 1; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; OTTHUMP00000030698; microtubule-associated proteins 1A/1B light chain 3
Accession Q91VR7

Image & description

Fig.1. Mouse Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A) Standard Curve.

Fig.1. Mouse Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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