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Mouse Microtubule-associated protein 1A (MAP1A) ELISA Kit

Mouse Microtubule-associated protein 1A (MAP1A) ELISA Kit

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Specification

Product name Mouse Microtubule-associated protein 1A (MAP1A) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Microtubule-associated protein 1A (MAP1A) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP1A in samples. An antibody specific for MAP1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAP1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MAP1A; FLJ77111; MAP1L; MTAP1A; microtubule-associated protein 1-like

Product Properties

Kit components • Mouse Microtubule-associated protein 1A microplate
• Mouse Microtubule-associated protein 1A standard
• Mouse Microtubule-associated protein 1A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Microtubule-associated protein 1A (MAP1A) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MAP1A. No significant cross-reactivity or interference between Mouse MAP1A and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background These proteins have been divided into 2 main groups by molecular mass, high molecular weight MAPs, which include MAP1A, MAP1B, and MAP2, and another group of intermediate-sized proteins, which include the abundant tau MAPs. MAP1B, also named MAP5, is a component of long cross-bridges between microtubules and is a filamentous molecule with a small spherical segment at one end.Lien et al. (1994) completely cloned and sequenced the human MAP1B gene. By comparison of human MAP1B with sequence databases, they identified a MAP1B-related gene that is probably the human homolog of rat MAP1A. The human MAP0A gene is expressed at high levels in brain and spinal cord and at much lower levels in muscle.
Gene ID 17754
Alternative MAP1A; FLJ77111; MAP1L; MTAP1A; microtubule-associated protein 1-like
Accession Q9QYR6

Image & description

Fig.1. Mouse Microtubule-associated protein 1A (MAP1A) Standard Curve.

Fig.1. Mouse Microtubule-associated protein 1A (MAP1A) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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