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Mouse Microcephalin (MCPH1) ELISA Kit

Mouse Microcephalin (MCPH1) ELISA Kit

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Specification

Product name Mouse Microcephalin (MCPH1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Microcephalin (MCPH1) ELISA Kit employs a two-site sandwich ELISA to quantitate MCPH1 in samples. An antibody specific for MCPH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCPH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCPH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCPH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MCPH1; BRIT1; FLJ12847; MCT; BRCT-repeat inhibitor of TERT expression 1; microcephalin; microcephaly; primary autosomal recessive 1

Product Properties

Kit components • Mouse Microcephalin microplate
• Mouse Microcephalin standard
• Mouse Microcephalin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Microcephalin (MCPH1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MCPH1. No significant cross-reactivity or interference between Mouse MCPH1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MCPH1 encodes a DNA damage response protein. The encoded protein may play a role in G2/M checkpoint arrest via maintenance of inhibitory phosphorylation of cyclin-dependent kinase 1. Mutations in MCPH1 have been associated with primary autosomal recessive microcephaly 1 and premature chromosome condensation syndrome. Alternatively spliced transcript variants have been described.
Gene ID 244329
Alternative MCPH1; BRIT1; FLJ12847; MCT; BRCT-repeat inhibitor of TERT expression 1; microcephalin; microcephaly; primary autosomal recessive 1
Accession Q7TT79

Image & description

Fig.1. Mouse Microcephalin (MCPH1) Standard Curve.

Fig.1. Mouse Microcephalin (MCPH1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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