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Mouse Methionine adenosyltransferase 2 subunit beta (MAT2B) ELISA Kit

Mouse Methionine adenosyltransferase 2 subunit beta (MAT2B) ELISA Kit

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Specification

Product name Mouse Methionine adenosyltransferase 2 subunit beta (MAT2B) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Methionine adenosyltransferase 2 subunit beta (MAT2B) ELISA Kit employs a two-site sandwich ELISA to quantitate MAT2B in samples. An antibody specific for MAT2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAT2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAT2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAT2B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MAT2B; MAT-II; MATIIbeta; MGC12237; Nbla02999; SDR23E1; TGR; beta regulatory subunit of methionine adenosyltransferase; dTDP-4-keto-6-deoxy-D-glucose 4-reductase; putative protein product of Nbla02999; short

Product Properties

Kit components • Mouse Methionine adenosyltransferase 2 subunit beta microplate
• Mouse Methionine adenosyltransferase 2 subunit beta standard
• Mouse Methionine adenosyltransferase 2 subunit beta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Methionine adenosyltransferase 2 subunit beta (MAT2B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MAT2B. No significant cross-reactivity or interference between Mouse MAT2B and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Methionine adenosyltransferase (MAT; EC 2.5.1.6) catalyzes the biosynthesis of S-adenosylmethionine (AdoMet) from methionine and ATP. MAT II is a broadly expressed MAT consisting of catalytic alpha and noncatalytic beta subunits encoded by MAT2A) and MAT2B, respectively.LeGros et al. (2000) designed degenerate PCR primers to tryptic peptide sequence of MAT2B purified from human lymphocytes. They amplified a partial cDNA from Jurkat cells and extended the cDNA using 3-prime and 5-prime RACE. A full-length MAT2B cDNA amplified from normal human peripheral blood mononuclear cell RNA encodes a 334-amino acid protein with a molecular mass of 38 kD.
Gene ID 108645
Alternative MAT2B; MAT-II; MATIIbeta; MGC12237; Nbla02999; SDR23E1; TGR; beta regulatory subunit of methionine adenosyltransferase; dTDP-4-keto-6-deoxy-D-glucose 4-reductase; putative protein product of Nbla02999; short
Accession Q99LB6

Image & description

Fig.1. Mouse Methionine adenosyltransferase 2 subunit beta (MAT2B) Standard Curve.

Fig.1. Mouse Methionine adenosyltransferase 2 subunit beta (MAT2B) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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