Login Register
English
0

Cart

$ 0

Mouse Mediator of RNA polymerase II transcription subunit 15 (MED15) ELISA Kit

Mouse Mediator of RNA polymerase II transcription subunit 15 (MED15) ELISA Kit

Views(91) Publications(0) Catalog no(KTE71071)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Mouse Mediator of RNA polymerase II transcription subunit 15 (MED15) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Mediator of RNA polymerase II transcription subunit 15 (MED15) ELISA Kit employs a two-site sandwich ELISA to quantitate MED15 in samples. An antibody specific for MED15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED15 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MED15; ARC105; CAG7A; CTG7A; DKFZp686A2214; DKFZp762B1216; FLJ42282; FLJ42935; PCQAP; TIG-1; TIG1; TNRC7; CTG repeat protein 7a; PC2 (positive cofactor 2; multiprotein complex) glutamine/Q-rich-associated p

Product Properties

Kit components • Mouse Mediator of RNA polymerase II transcription subunit 15 microplate
• Mouse Mediator of RNA polymerase II transcription subunit 15 standard
• Mouse Mediator of RNA polymerase II transcription subunit 15 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Mediator of RNA polymerase II transcription subunit 15 (MED15) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MED15. No significant cross-reactivity or interference between Mouse MED15 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway.
Alternative MED15; ARC105; CAG7A; CTG7A; DKFZp686A2214; DKFZp762B1216; FLJ42282; FLJ42935; PCQAP; TIG-1; TIG1; TNRC7; CTG repeat protein 7a; PC2 (positive cofactor 2; multiprotein complex) glutamine/Q-rich-associated p
Accession Q924H2

Image & description

Fig.1. Mouse Mediator of RNA polymerase II transcription subunit 15 (MED15) Standard Curve.

Fig.1. Mouse Mediator of RNA polymerase II transcription subunit 15 (MED15) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Mouse Mediator of RNA polymerase II transcription subunit 15 (MED15) ELISA Kit”