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Mouse Mediator of RNA polymerase II transcription subunit 14 (MED14) ELISA Kit

Mouse Mediator of RNA polymerase II transcription subunit 14 (MED14) ELISA Kit

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Specification

Product name Mouse Mediator of RNA polymerase II transcription subunit 14 (MED14) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Mediator of RNA polymerase II transcription subunit 14 (MED14) ELISA Kit employs a two-site sandwich ELISA to quantitate MED14 in samples. An antibody specific for MED14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MED14; CRSP150; CRSP2; CSRP; CXorf4; DRIP150; EXLM1; MGC104513; RGR1; TRAP170; cofactor required for Sp1 transcriptional activation; subunit 2 (150kD); cofactor required for Sp1 transcriptional activation;

Product Properties

Kit components • Mouse Mediator of RNA polymerase II transcription subunit 14 microplate
• Mouse Mediator of RNA polymerase II transcription subunit 14 standard
• Mouse Mediator of RNA polymerase II transcription subunit 14 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Mediator of RNA polymerase II transcription subunit 14 (MED14) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MED14. No significant cross-reactivity or interference between Mouse MED14 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by MED14 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. MED14 is known to escape chromosome X-inactivation.
Gene ID 26896
Alternative MED14; CRSP150; CRSP2; CSRP; CXorf4; DRIP150; EXLM1; MGC104513; RGR1; TRAP170; cofactor required for Sp1 transcriptional activation; subunit 2 (150kD); cofactor required for Sp1 transcriptional activation;
Accession A2ABV5

Image & description

Fig.1. Mouse Mediator of RNA polymerase II transcription subunit 14 (MED14) Standard Curve.

Fig.1. Mouse Mediator of RNA polymerase II transcription subunit 14 (MED14) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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