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Mouse Matrix metalloproteinase 8/Neutrophil collagenase (MMP8) ELISA Kit

Mouse Matrix metalloproteinase 8/Neutrophil collagenase (MMP8) ELISA Kit

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Specification

Product name Mouse Matrix metalloproteinase 8/Neutrophil collagenase (MMP8) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Matrix metalloproteinase 8/Neutrophil collagenase (MMP8) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP8 in samples. An antibody specific for MMP8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMP8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MMP8; CLG1; HNC; PMNL-CL; PMNL collagenase; matrix metalloproteinase 8; matrix metalloproteinase 8 (neutrophil collagenase)

Product Properties

Kit components • Mouse Matrix metalloproteinase 8/Neutrophil collagenase microplate
• Mouse Matrix metalloproteinase 8/Neutrophil collagenase standard
• Mouse Matrix metalloproteinase 8/Neutrophil collagenase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Matrix metalloproteinase 8/Neutrophil collagenase (MMP8) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MMP8. No significant cross-reactivity or interference between Mouse MMP8 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Mmp8 encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme that degrades types I, II and III collagens. Mice lacking the encoded protein exhibit abnormalities in the inflammatory responses to various agents. Mmp8 is located in a cluster of other matrix metalloproteinase genes on chromosome 9.
Gene ID 17394
Alternative MMP8; CLG1; HNC; PMNL-CL; PMNL collagenase; matrix metalloproteinase 8; matrix metalloproteinase 8 (neutrophil collagenase)
Accession O70138

Image & description

Fig.1. Mouse Matrix metalloproteinase 8/Neutrophil collagenase (MMP8) Standard Curve.

Fig.1. Mouse Matrix metalloproteinase 8/Neutrophil collagenase (MMP8) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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