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Mouse Matrix metalloproteinase-20 (MMP20) ELISA Kit

Mouse Matrix metalloproteinase-20 (MMP20) ELISA Kit

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Specification

Product name Mouse Matrix metalloproteinase-20 (MMP20) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Matrix metalloproteinase-20 (MMP20) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP20 in samples. An antibody specific for MMP20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMP20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP20 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MMP20; AI2A2; MMP-20; enamel metalloproteinase; matrix metalloproteinase 20; matrix metalloproteinase 20 (enamelysin)

Product Properties

Kit components • Mouse Matrix metalloproteinase-20 microplate
• Mouse Matrix metalloproteinase-20 standard
• Mouse Matrix metalloproteinase-20 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Matrix metalloproteinase-20 (MMP20) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MMP20. No significant cross-reactivity or interference between Mouse MMP20 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Mmp20 encodes a member of the matrix metalloproteinase family of zinc-dependent endopeptidases capable of degrading extracellular matrix proteins. Mmp20 is expressed specifically in the ameloblasts and odontoblasts, and the encoded protein is an inactive zymogen that requires proteolytic removal of a N-terminal propeptide to become enzymatically active. Mice lacking the encoded protein display an amelogenesis imperfecta phenotype.
Gene ID 30800
Alternative MMP20; AI2A2; MMP-20; enamel metalloproteinase; matrix metalloproteinase 20; matrix metalloproteinase 20 (enamelysin)
Accession P57748

Image & description

Fig.1. Mouse Matrix metalloproteinase-20 (MMP20) Standard Curve.

Fig.1. Mouse Matrix metalloproteinase-20 (MMP20) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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