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Mouse Matrix metallopeptidase 7 (MMP7) ELISA Kit

Mouse Matrix metallopeptidase 7 (MMP7) ELISA Kit

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Specification

Product name Mouse Matrix metallopeptidase 7 (MMP7) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Matrix metallopeptidase 7 (MMP7) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP7 in samples. An antibody specific for MMP7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMP7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MMP7; MMP-7; MPSL1; PUMP-1; matrin; matrix metalloproteinase 7; matrix metalloproteinase 7 (matrilysin; uterine); uterine matrilysin

Product Properties

Kit components • Mouse Matrix metallopeptidase 7 microplate
• Mouse Matrix metallopeptidase 7 standard
• Mouse Matrix metallopeptidase 7 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Matrix metallopeptidase 7 (MMP7) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MMP7. No significant cross-reactivity or interference between Mouse MMP7 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MMP7, also known as Matrilysin, is the smallest member of the MMP-family, it is a zinc-dependent metalloproteinase and is overexpressed in colon cancer and many other human cancers. MMP7 is a secreted matrix metalloproteinase, which contributes to angiogenesis by breaking down basement membranes. Matrix Metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components.MMP7 is responsible for hydrolyzing proteoglycans and ECM glycoproteins. It is expressed in normal cells, specifically glandular epithelial cells. MMP7, is thought to play an important role in the early steps of tumor progression. Expression of MMP2, MMP7 and MMP11 is greater in pancreatic carcinoma than in normal pancreas.
Alternative MMP7; MMP-7; MPSL1; PUMP-1; matrin; matrix metalloproteinase 7; matrix metalloproteinase 7 (matrilysin; uterine); uterine matrilysin
Accession Q10738

Image & description

Fig.1. Mouse Matrix metallopeptidase 7 (MMP7) Standard Curve.

Fig.1. Mouse Matrix metallopeptidase 7 (MMP7) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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