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Mouse MANSC domain-containing protein 1 (MANSC1) ELISA Kit

Mouse MANSC domain-containing protein 1 (MANSC1) ELISA Kit

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Specification

Product name Mouse MANSC domain-containing protein 1 (MANSC1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse MANSC domain-containing protein 1 (MANSC1) ELISA Kit employs a two-site sandwich ELISA to quantitate MANSC1 in samples. An antibody specific for MANSC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMANSC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MANSC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MANSC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MANSC1; 9130403P13Rik; FLJ10298; LOH12CR3;

Product Properties

Kit components • Mouse MANSC domain-containing protein 1 microplate
• Mouse MANSC domain-containing protein 1 standard
• Mouse MANSC domain-containing protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse MANSC domain-containing protein 1 (MANSC1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MANSC1. No significant cross-reactivity or interference between Mouse MANSC1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MANSC1 Contains 1 MANSC domain. MANSC: a seven-cysteine-containing domain present in animal membrane and extracellular proteins.MANSC (motif at N terminus with seven cysteines) is a novel domain with a well-conserved seven-cysteine motif that is present at the N terminus of membrane and extracellular proteins, including low-density lipoprotein receptor-related protein 11 (LRP-11), hepatocyte growth factor activator inhibitor 1 (HAI-1) and some uncharacterized proteins encoded by multicellular animals from Mollusca to Chordata. the MANSC domain in HAI-2 might function through binding with hepatocyte growth factor activator and matriptase.
Gene ID 67729
Alternative MANSC1; 9130403P13Rik; FLJ10298; LOH12CR3;
Accession Q9CR33

Image & description

Fig.1. Mouse MANSC domain-containing protein 1 (MANSC1) Standard Curve.

Fig.1. Mouse MANSC domain-containing protein 1 (MANSC1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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