Login Register
English
0

Cart

$ 0

Mouse Mannan-binding lectin serine protease 1 (MASP1) ELISA Kit

Mouse Mannan-binding lectin serine protease 1 (MASP1) ELISA Kit

Views(146) Publications(0) Catalog no(KTE71122)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Mouse Mannan-binding lectin serine protease 1 (MASP1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Mannan-binding lectin serine protease 1 (MASP1) ELISA Kit employs a two-site sandwich ELISA to quantitate MASP1 in samples. An antibody specific for MASP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMASP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MASP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MASP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MASP1; CRARF; CRARF1; DKFZp686I01199; FLJ26383; MASP; MGC126283; MGC126284; PRSS5; RaRF; Ra-reactive factor serine protease p100; manan-binding lectin serine protease-1; mannan-binding lectin serine protease

Product Properties

Kit components • Mouse Mannan-binding lectin serine protease 1 microplate
• Mouse Mannan-binding lectin serine protease 1 standard
• Mouse Mannan-binding lectin serine protease 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Mannan-binding lectin serine protease 1 (MASP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MASP1. No significant cross-reactivity or interference between Mouse MASP1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Mannose-associated serine protease 1 (MASP1) is involved in the lectin pathway of the complement system and is responsible for cleaving C4 and C2 to form C4b2a, a C3-convertase.Mannose-binding lectin (MBL) is a serum component which participates in innate immunity by activating complement via a novel pathway. Human MBL forms complexes with two types of serine proteases termed MASP (MBL-associated serine protease). These two proteases, MASP1 and MASP2, are structurally similar to one another as well as to C1r and C1s. Together, MASP, C1r and C1s constitute a novel serine protease family. It is likely that human MASP1 is able to activate C3, while human MASP2 cleaves C4, although further functional studies are required to confirm this.
Gene ID 17174
Alternative MASP1; CRARF; CRARF1; DKFZp686I01199; FLJ26383; MASP; MGC126283; MGC126284; PRSS5; RaRF; Ra-reactive factor serine protease p100; manan-binding lectin serine protease-1; mannan-binding lectin serine protease
Accession P98064

Image & description

Fig.1. Mouse Mannan-binding lectin serine protease 1 (MASP1) Standard Curve.

Fig.1. Mouse Mannan-binding lectin serine protease 1 (MASP1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Mouse Mannan-binding lectin serine protease 1 (MASP1) ELISA Kit”