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Mouse Malate Dehydrogenase (MDH) ELISA Kit

Mouse Malate Dehydrogenase (MDH) ELISA Kit

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Specification

Product name Mouse Malate Dehydrogenase (MDH) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Malate Dehydrogenase (MDH) ELISA Kit employs a two-site sandwich ELISA to quantitate MDH1 in samples. An antibody specific for MDH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMDH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MDH1; MDH-s; MDHA; MGC:1375; MOR2; cytosolic malate dehydrogenase; soluble malate dehydrogenase

Product Properties

Kit components • Mouse Malate Dehydrogenase microplate
• Mouse Malate Dehydrogenase standard
• Mouse Malate Dehydrogenase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Malate Dehydrogenase (MDH) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MDH1. No significant cross-reactivity or interference between Mouse MDH1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Mdh1 encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. Mdh1 encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. A pseudogene has been identified on chromosomes 12.
Gene ID 17449
Alternative MDH1; MDH-s; MDHA; MGC:1375; MOR2; cytosolic malate dehydrogenase; soluble malate dehydrogenase
Accession P14152

Image & description

Fig.1. Mouse Malate Dehydrogenase (MDH) Standard Curve.

Fig.1. Mouse Malate Dehydrogenase (MDH) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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