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Mouse Lysosome-associated membrane glycoprotein 3 (LAMP3) ELISA Kit

Mouse Lysosome-associated membrane glycoprotein 3 (LAMP3) ELISA Kit

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Specification

Product name Mouse Lysosome-associated membrane glycoprotein 3 (LAMP3) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Lysosome-associated membrane glycoprotein 3 (LAMP3) ELISA Kit employs a two-site sandwich ELISA to quantitate LAMP3 in samples. An antibody specific for LAMP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAMP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAMP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative LAMP3; CD208; DC-LAMP; DCLAMP; LAMP; TSC403;

Product Properties

Kit components • Mouse Lysosome-associated membrane glycoprotein 3 microplate
• Mouse Lysosome-associated membrane glycoprotein 3 standard
• Mouse Lysosome-associated membrane glycoprotein 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Lysosome-associated membrane glycoprotein 3 (LAMP3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LAMP3. No significant cross-reactivity or interference between Mouse LAMP3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background LAMP3 may play a role in dendritic cell function and in adaptive immunity. By screening a DC subtraction library, de Saint-Vis et al. (1998) isolated a cDNA encoding DCLAMP. Sequence analysis predicted that the 416-amino acid type I integral membrane protein has an N-terminal signal peptide, followed by a 381-amino acid extracellular domain, a hydrophobic transmembrane domain, and a 10-amino acid cytoplasmic domain containing a conserved GY lysosomal targeting motif, characteristic of LAMP family members. The DCLAMP protein contains multiple N- and O-glycosylation sites. Northern blot and RT-PCR analysis revealed expression of a 3.2-kb transcript in appendix, thymus, lymph node, lung, and weakly in spleen, as well as in dendritic cell lines; expression was weak or undetectable in other cell lines.
Gene ID 239739
Alternative LAMP3; CD208; DC-LAMP; DCLAMP; LAMP; TSC403;
Accession Q7TST5

Image & description

Fig.1. Mouse Lysosome-associated membrane glycoprotein 3 (LAMP3) Standard Curve.

Fig.1. Mouse Lysosome-associated membrane glycoprotein 3 (LAMP3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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