|Product name||Mouse Low-density lipoprotein receptor class A domain-containing protein 3 (LDLRAD3) ELISA Kit|
|Applications notes||This Mouse Low-density lipoprotein receptor class A domain-containing protein 3 (LDLRAD3) ELISA Kit employs a two-site sandwich ELISA to quantitate LDLRAD3 in samples. An antibody specific for LDLRAD3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLDLRAD3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LDLRAD3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LDLRAD3 bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Mouse Low-density lipoprotein receptor class A domain-containing protein 3 microplate
• Mouse Low-density lipoprotein receptor class A domain-containing protein 3 standard
• Mouse Low-density lipoprotein receptor class A domain-containing protein 3 detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Mouse Low-density lipoprotein receptor class A domain-containing protein 3 (LDLRAD3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LDLRAD3. No significant cross-reactivity or interference between Mouse LDLRAD3 and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||LDLRAD3 Belongs to the LDLR family. Contains 3 LDL-receptor class A domains.The low density lipoprotein receptor gene family code for a class of structurally closely related cell surface receptors that fulfill diverse biological functions in different organs, tissues, and cell types. The role that is most commonly associated with this evolutionarily ancient family is cholesterol homeostasis (maintenance of appropriate concentration of cholesterol). In humans, excess cholesterol in the blood is captured by low-density lipoprotein (LDL) and removed in the liver by the endocytosis of the LDL receptor. Recent evidence indicates that the members of the LDL receptor gene family are active in the cell signalling pathways between specialized cells in many, if not all, multicellular organisms.|
Fig.1. Mouse Low-density lipoprotein receptor class A domain-containing protein 3 (LDLRAD3) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.