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Mouse Low density lipoprotein receptor adapter protein 1 (LDLRAP1) ELISA Kit

Mouse Low density lipoprotein receptor adapter protein 1 (LDLRAP1) ELISA Kit

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Specification

Product name Mouse Low density lipoprotein receptor adapter protein 1 (LDLRAP1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Low density lipoprotein receptor adapter protein 1 (LDLRAP1) ELISA Kit employs a two-site sandwich ELISA to quantitate LDLRAP1 in samples. An antibody specific for LDLRAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLDLRAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LDLRAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LDLRAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative LDLRAP1; RP11-70P17.2; ARH; ARH1; ARH2; DKFZp586D0624; FHCB1; FHCB2; MGC34705; LDL receptor adaptor protein; autosomal recessive hypercholesterolemia protein

Product Properties

Kit components • Mouse Low density lipoprotein receptor adapter protein 1 microplate
• Mouse Low density lipoprotein receptor adapter protein 1 standard
• Mouse Low density lipoprotein receptor adapter protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Low density lipoprotein receptor adapter protein 1 (LDLRAP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LDLRAP1. No significant cross-reactivity or interference between Mouse LDLRAP1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background LDLRAP1 is a protein encoded by this gene is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. Mutations in this gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia.Expressed at high levels in the kidney, liver, and placenta, with lower levels detectable in brain, heart, muscle, colon, spleen, intestine, lung, and leukocytes.Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits.
Gene ID 100017
Alternative LDLRAP1; RP11-70P17.2; ARH; ARH1; ARH2; DKFZp586D0624; FHCB1; FHCB2; MGC34705; LDL receptor adaptor protein; autosomal recessive hypercholesterolemia protein
Accession Q8C142

Image & description

Fig.1. Mouse Low density lipoprotein receptor adapter protein 1 (LDLRAP1) Standard Curve.

Fig.1. Mouse Low density lipoprotein receptor adapter protein 1 (LDLRAP1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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