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Mouse Long-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADL) ELISA Kit

Mouse Long-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADL) ELISA Kit

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Specification

Product name Mouse Long-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADL) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Long-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADL) ELISA Kit employs a two-site sandwich ELISA to quantitate ACADL in samples. An antibody specific for ACADL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACADL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACADL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACADL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ACADL; ACAD4; FLJ94052; LCAD; long-chain acyl-CoA dehydrogenase

Product Properties

Kit components • Mouse Long-chain specific acyl-CoA dehydrogenase, mitochondrial microplate
• Mouse Long-chain specific acyl-CoA dehydrogenase, mitochondrial standard
• Mouse Long-chain specific acyl-CoA dehydrogenase, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Long-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADL) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ACADL. No significant cross-reactivity or interference between Mouse ACADL and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ACADL is a protein belongs to the acyl-CoA dehydrogenase family, which is a family of mitochondrial flavoenzymes involved in fatty acid and branched chain amino-acid metabolism. This protein is one of the four enzymes that catalyze the initial step of mitochondrial beta-oxidation of straight-chain fatty acid. Defects in this gene are the cause of long-chain acyl-CoA dehydrogenase (LCAD) deficiency, leading to nonketotic hypoglycemia. Impaired ACADL activity results in non-ketotic hypoglycemia, hypotonia, muscle weakness and episodes of cardiorespiratory arrest associated with fasting. A number of straight-chain acyl-CoA dehydrogenases of different substrate specificities are present in mammalian tissues.
Gene ID 11363
Alternative ACADL; ACAD4; FLJ94052; LCAD; long-chain acyl-CoA dehydrogenase
Accession P51174

Image & description

Fig.1. Mouse Long-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADL) Standard Curve.

Fig.1. Mouse Long-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADL) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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