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Mouse Lipolysis-stimulated lipoprotein receptor (LSR) ELISA Kit

Mouse Lipolysis-stimulated lipoprotein receptor (LSR) ELISA Kit

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Specification

Product name Mouse Lipolysis-stimulated lipoprotein receptor (LSR) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Lipolysis-stimulated lipoprotein receptor (LSR) ELISA Kit employs a two-site sandwich ELISA to quantitate LSR in samples. An antibody specific for LSR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLSR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LSR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LSR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative LSR; ILDR3; LISCH7; MGC10659; MGC48312; MGC48503; LISCH protein; immunoglobulin-like domain containing receptor 3; lipolysis-stimulated receptor; lipolysis-stimulated remnant; liver-specific bHLH-Zip transcr

Product Properties

Kit components • Mouse Lipolysis-stimulated lipoprotein receptor microplate
• Mouse Lipolysis-stimulated lipoprotein receptor standard
• Mouse Lipolysis-stimulated lipoprotein receptor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Lipolysis-stimulated lipoprotein receptor (LSR) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LSR. No significant cross-reactivity or interference between Mouse LSR and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background LSR is a multimeric protein complex in the liver that undergoes conformational changes upon binding of free fatty acids, thereby revealing a binding site (s) that recognizes both apoB and apoE. Complete inactivation of the LSR gene is embryonic lethal in mice.LSR cooperates with the LDL receptor in the final hepatic processing of apoB-containing lipoproteins and represents a novel therapeutic target for the treatment of hyperlipidemia associated with obesity and atherosclerosis. The lipolysis-stimulated receptor (LSR) is a lipoprotein receptor primarily expressed in the liver and activated by free fatty acids. Antibodies inhibiting LSR functions showed that the receptor is a heterotrimer or tetramer consisting of 68-kDa (alpha) and 55-kDa (beta) subunits associated through disulfide bridges.
Gene ID 54135
Alternative LSR; ILDR3; LISCH7; MGC10659; MGC48312; MGC48503; LISCH protein; immunoglobulin-like domain containing receptor 3; lipolysis-stimulated receptor; lipolysis-stimulated remnant; liver-specific bHLH-Zip transcr
Accession Q99KG5

Image & description

Fig.1. Mouse Lipolysis-stimulated lipoprotein receptor (LSR) Standard Curve.

Fig.1. Mouse Lipolysis-stimulated lipoprotein receptor (LSR) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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