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Mouse LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) ELISA Kit

Mouse LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) ELISA Kit

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Specification

Product name Mouse LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) ELISA Kit employs a two-site sandwich ELISA to quantitate LIMS1 in samples. An antibody specific for LIMS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLIMS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LIMS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LIMS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative LIMS1; PINCH; PINCH1; OTTHUMP00000161608

Product Properties

Kit components • Mouse LIM and senescent cell antigen-like-containing domain protein 1 microplate
• Mouse LIM and senescent cell antigen-like-containing domain protein 1 standard
• Mouse LIM and senescent cell antigen-like-containing domain protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LIMS1. No significant cross-reactivity or interference between Mouse LIMS1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background LIMS1 is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.While ILK depletion reduced AKT phosphorylation on ser473, PINCH1 depletion reduced AKT phosphorylation on both ser473 and thr308. PINCH1 also regulated ILK protein levels.
Gene ID 110829
Alternative LIMS1; PINCH; PINCH1; OTTHUMP00000161608
Accession Q99JW4

Image & description

Fig.1. Mouse LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) Standard Curve.

Fig.1. Mouse LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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