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Mouse Leukocyte cell derived chemotaxin 1 (LECT1) ELISA Kit

Mouse Leukocyte cell derived chemotaxin 1 (LECT1) ELISA Kit

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Specification

Product name Mouse Leukocyte cell derived chemotaxin 1 (LECT1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Leukocyte cell derived chemotaxin 1 (LECT1) ELISA Kit employs a two-site sandwich ELISA to quantitate LECT1 in samples. An antibody specific for LECT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLECT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LECT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LECT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative LECT1; RP11-93H24.1; BRICD3; CHM-I; CHM1; BRICHOS domain containing 3; chondromodulin I

Product Properties

Kit components • Mouse Leukocyte cell derived chemotaxin 1 microplate
• Mouse Leukocyte cell derived chemotaxin 1 standard
• Mouse Leukocyte cell derived chemotaxin 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Leukocyte cell derived chemotaxin 1 (LECT1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LECT1. No significant cross-reactivity or interference between Mouse LECT1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background LECT1 is a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. The N-terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein although no biological activity has yet been defined for it. The C-terminus of the precursor protein contains a 25 kDa mature protein called leukocyte cell-derived chemotaxin-1 or chondromodulin-1. The mature protein promotes chondrocyte growth and inhibits angiogenesis. LECT1 expressed in the avascular zone of prehypertrophic cartilage and its expression decreases during chondrocyte hypertrophy and vascular invasion. The mature protein likely plays a role in endochondral bone development by permitting cartilaginous anlagen to be vascularized and replaced by bone.
Gene ID 16840
Alternative LECT1; RP11-93H24.1; BRICD3; CHM-I; CHM1; BRICHOS domain containing 3; chondromodulin I
Accession Q9Z1F6

Image & description

Fig.1. Mouse Leukocyte cell derived chemotaxin 1 (LECT1) Standard Curve.

Fig.1. Mouse Leukocyte cell derived chemotaxin 1 (LECT1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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