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Mouse Leucine-rich repeat LGI family member 3 (LGI3) ELISA Kit

Mouse Leucine-rich repeat LGI family member 3 (LGI3) ELISA Kit

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Specification

Product name Mouse Leucine-rich repeat LGI family member 3 (LGI3) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Leucine-rich repeat LGI family member 3 (LGI3) ELISA Kit employs a two-site sandwich ELISA to quantitate LGI3 in samples. An antibody specific for LGI3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGI3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGI3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGI3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative LGI3; LGIL4;

Product Properties

Kit components • Mouse Leucine-rich repeat LGI family member 3 microplate
• Mouse Leucine-rich repeat LGI family member 3 standard
• Mouse Leucine-rich repeat LGI family member 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Leucine-rich repeat LGI family member 3 (LGI3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LGI3. No significant cross-reactivity or interference between Mouse LGI3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The deduced 549-amino acid protein contains a N-terminal signal peptide, a putative transmembrane region, and 1 partial and 4 complete leucine-rich repeats (LRRs). The LRRs are flanked on both sides by cysteine-rich regions, and the N-terminal cysteine-rich region contains a motif conserved in LGIs. LGI3 shares significant similarity with LGI1, LGI2, and LGI4. Semiquantitative PCR detected LGI3 expression in all tissues examined, with highest levels in brain and lung. By in situ hybridization of adult mouse brain, Senechal et al. (2005) showed that Lgi3 was expressed at low levels in most brain regions with high levels in the facial nerve nucleus. Lgi3 was secreted in transfected 293T cells.
Gene ID 213469
Alternative LGI3; LGIL4;
Accession Q8K406

Image & description

Fig.1. Mouse Leucine-rich repeat LGI family member 3 (LGI3) Standard Curve.

Fig.1. Mouse Leucine-rich repeat LGI family member 3 (LGI3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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