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Mouse Legumain (LGMN) ELISA Kit

Mouse Legumain (LGMN) ELISA Kit

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Specification

Product name Mouse Legumain (LGMN) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Legumain (LGMN) ELISA Kit employs a two-site sandwich ELISA to quantitate LGMN in samples. An antibody specific for LGMN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGMN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGMN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGMN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative LGMN; AEP; LGMN1; PRSC1; asparaginyl endopeptidase; cysteine protease 1; protease; cysteine; 1 (legumain)

Product Properties

Kit components • Mouse Legumain microplate
• Mouse Legumain standard
• Mouse Legumain detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Legumain (LGMN) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LGMN. No significant cross-reactivity or interference between Mouse LGMN and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Legumain is a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined.
Gene ID 19141
Alternative LGMN; AEP; LGMN1; PRSC1; asparaginyl endopeptidase; cysteine protease 1; protease; cysteine; 1 (legumain)
Accession O89017

Image & description

Fig.1. Mouse Legumain (LGMN) Standard Curve.

Fig.1. Mouse Legumain (LGMN) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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