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Mouse Left-right determination factor 1 (LEFTY1) ELISA Kit

Mouse Left-right determination factor 1 (LEFTY1) ELISA Kit

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Specification

Product name Mouse Left-right determination factor 1 (LEFTY1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Left-right determination factor 1 (LEFTY1) ELISA Kit employs a two-site sandwich ELISA to quantitate LEFTY1 in samples. An antibody specific for LEFTY1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLEFTY1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LEFTY1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LEFTY1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative LEFTY1; LEFTB; LEFTYB; left-right determination; factor B

Product Properties

Kit components • Mouse Left-right determination factor 1 microplate
• Mouse Left-right determination factor 1 standard
• Mouse Left-right determination factor 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Left-right determination factor 1 (LEFTY1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LEFTY1. No significant cross-reactivity or interference between Mouse LEFTY1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Vertebrates exhibit numerous left-right (L-R) asymmetries, such as the position of the heart and spleen on the left side. L-R polarity is determined early during development, around the presomite stage in mammals. Several genes have been shown to be expressed asymmetrically relative to the L-R axis. Meno et al. (1996, 1997) identified 2 transforming growth factor-beta-related genes, Lefty1 and Lefty2, that are expressed on the left side of developing mouse embryos.Kosaki et al. (1999) characterized 2 homologs of the murine Lefty1 and Lefty2 genes, which they designated LEFTY B and LEFTY A, respectively. Both genes encode proteins with 366 amino acids. The deduced amino acid sequences of LEFTY B and LEFTY A are more similar to each other than to mouse Lefty1 or Lefty2.
Gene ID 13590
Alternative LEFTY1; LEFTB; LEFTYB; left-right determination; factor B
Accession Q64280

Image & description

Fig.1. Mouse Left-right determination factor 1 (LEFTY1) Standard Curve.

Fig.1. Mouse Left-right determination factor 1 (LEFTY1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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