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Mouse Krueppel-like factor 1 (KLF1) ELISA Kit

Mouse Krueppel-like factor 1 (KLF1) ELISA Kit

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Specification

Product name Mouse Krueppel-like factor 1 (KLF1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Krueppel-like factor 1 (KLF1) ELISA Kit employs a two-site sandwich ELISA to quantitate KLF1 in samples. An antibody specific for KLF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative KLF1; EKLF; INLU; erythroid Kruppel-like factor; erythroid-specific transcription factor EKLF; monoclonal antibody A3D8

Product Properties

Kit components • Mouse Krueppel-like factor 1 microplate
• Mouse Krueppel-like factor 1 standard
• Mouse Krueppel-like factor 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Krueppel-like factor 1 (KLF1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse KLF1. No significant cross-reactivity or interference between Mouse KLF1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Erythroid Krüppel-like Factor (EKLF) is a transcription factor that is necessary for the proper maturation of erythroid (red blood) cells. The molecule has two domains; the transactivation domain and the chromatin-remodeling domain. The carboxyl (C) terminal is composed of three C2H2 zinc fingers that binds to DNA, and the amino (N) terminus is proline rich and acidic. The gene for EKLF is on the human chromosome 19 and on mouse chromosome 8. EKLF deficient (knockout) mouse embryos exhibit a lethal anemic phenotype, fail to promote the transcription of adult β globin, and die by embryonic day 14. On the other hand, over-expression of EKLF results in a reduction of the number of circulating platelets and hastens the onset of β globin gene.
Alternative KLF1; EKLF; INLU; erythroid Kruppel-like factor; erythroid-specific transcription factor EKLF; monoclonal antibody A3D8
Accession P46099

Image & description

Fig.1. Mouse Krueppel-like factor 1 (KLF1) Standard Curve.

Fig.1. Mouse Krueppel-like factor 1 (KLF1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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