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Mouse Keratinocyte Growth Factor (KGF/FGF-7) ELISA Kit

Mouse Keratinocyte Growth Factor (KGF/FGF-7) ELISA Kit

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Specification

Product name Mouse Keratinocyte Growth Factor (KGF/FGF-7) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Keratinocyte Growth Factor (KGF/FGF-7) ELISA Kit employs a two-site sandwich ELISA to quantitate FGF7 in samples. An antibody specific for FGF7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFGF7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative FGF7; HBGF-7; KGF; fibroblast growth factor 7; heparin-binding growth factor 7; keratinocyte growth factor

Product Properties

Kit components • Mouse Keratinocyte Growth Factor microplate
• Mouse Keratinocyte Growth Factor standard
• Mouse Keratinocyte Growth Factor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Keratinocyte Growth Factor (KGF/FGF-7) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse FGF7. No significant cross-reactivity or interference between Mouse FGF7 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Keratinocyte growth factor is a protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.
Gene ID 14178
Alternative FGF7; HBGF-7; KGF; fibroblast growth factor 7; heparin-binding growth factor 7; keratinocyte growth factor
Accession P36363

Image & description

Fig.1. Mouse Keratinocyte Growth Factor (KGF/FGF-7) Standard Curve.

Fig.1. Mouse Keratinocyte Growth Factor (KGF/FGF-7) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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