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Mouse IL-4 protein

Mouse IL-4 protein

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Specification

Product name Mouse IL-4 protein
Sequence Amino acid sequence derived from mouse IL4 (NP_067258.1) (His23-Ser140) was expressed with a N-terminal Met.
Activity Measured in a cell proliferation assay using HT-2 mouse T cells. The ED50 for this effect is typically 0.4-2 ng/mL. The ED50 for this effect is typically 0.4-2 ng/mL.
Protein length The recombinant mouse IL4 consists of 119 amino acids and predicts a molecular mass of 13.4 KDa. It migrates as an approximately 14 KDa band in SDS-PAGE under reducing conditions.
Preparation method E. coli
Purity > 90 % as determined by SDS-PAGE
Alternative BSF-1; Il-4; Interleukin-4; IL4

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4.
Molecular weight 13.4 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse IL4 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Mouse IL4 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as of other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibites in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and of the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.
Gene ID 16189
Alternative BSF-1; Il-4; Interleukin-4; IL4
Accession NP_067258.1

Image & description

Fig.SDS-PAGE analysis of Mouse IL-4 protein.

Fig.SDS-PAGE analysis of Mouse IL-4 protein.

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