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Mouse IL-17 protein

Mouse IL-17 protein

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Specification

Product name Mouse IL-17 protein
Sequence Amino acid sequence derived from mature form of mouse IL17a (Q62386) (Thr 22-Ala158) was expressed and purified.
Activity Measured by its binding ability in a functional ELISA. Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells in the presense of 20 ng/mL TNFα. The ED50 for this effect is 0.15-0.6 ng/mL. 2. Immobilized recombinant mouse IL17a (Cat:51065-MNAE) at 10 μg/mL (100 μl/well) can bind human IL17Ra-Fc2h (Cat:10895-H03H) with a linear range of 16-500 ng/mL.
Protein length The recombinant mouse IL17a consists of 138 amino acids and predicts a molecular mass of 15.5 KDa. It migrates as an approximately 16 KDa band in SDS-PAGE under reducing conditions.
Preparation method E. coli
Purity > 97 % as determined by SDS-PAGE
Alternative Ctla-8; Ctla8; IL-17; IL-17A; Il17; Interleukin-17A; IL17A

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4
Molecular weight 15.5 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse IL17 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Mouse IL17 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background IL17, also known as IL17a, is a cytokine belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Gene ID 16171
Alternative Ctla-8; Ctla8; IL-17; IL-17A; Il17; Interleukin-17A; IL17A
Accession Q62386

Image & description

Fig.SDS-PAGE analysis of Mouse IL-17 protein.

Fig.SDS-PAGE analysis of Mouse IL-17 protein.

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