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Mouse Homeobox protein Meis3 (MEIS3) ELISA Kit

Mouse Homeobox protein Meis3 (MEIS3) ELISA Kit

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Specification

Product name Mouse Homeobox protein Meis3 (MEIS3) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Homeobox protein Meis3 (MEIS3) ELISA Kit employs a two-site sandwich ELISA to quantitate MEIS3 in samples. An antibody specific for MEIS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMEIS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MEIS3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MEIS3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MEIS3; DKFZp547H236; MRG2; Meis1; myeloid ecotropic viral integration site 1 homolog 3; homeobox protein Meis3

Product Properties

Kit components • Mouse Homeobox protein Meis3 microplate
• Mouse Homeobox protein Meis3 standard
• Mouse Homeobox protein Meis3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Homeobox protein Meis3 (MEIS3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MEIS3. No significant cross-reactivity or interference between Mouse MEIS3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoding Meis3 belongs to the three amino acid loop extension family of homeodomain transcription factors, which play essential roles in many embryonic processes. These proteins are characterized by an atypical homeodomain containing a three amino acid loop extension between helices 1 and 2. Expression of Meis3 begins during the compaction stage of embryogenesis and continues into the blastocyst stage. This gene is also expressed in pancreatic islet cells and beta-cells and regulates beta-cell survival. Alternative splicing results in multiple transcript variants.
Gene ID 17537
Alternative MEIS3; DKFZp547H236; MRG2; Meis1; myeloid ecotropic viral integration site 1 homolog 3; homeobox protein Meis3
Accession P97368

Image & description

Fig.1. Mouse Homeobox protein Meis3 (MEIS3) Standard Curve.

Fig.1. Mouse Homeobox protein Meis3 (MEIS3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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