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Mouse Histone lysine demethylase PHF8 (PHF8) ELISA Kit

Mouse Histone lysine demethylase PHF8 (PHF8) ELISA Kit

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Specification

Product name Mouse Histone lysine demethylase PHF8 (PHF8) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Histone lysine demethylase PHF8 (PHF8) ELISA Kit employs a two-site sandwich ELISA to quantitate PHF8 in samples. An antibody specific for PHF8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPHF8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PHF8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PHF8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PHF8; RP13-444K19.2; DKFZp686E0868; JHDM1F; KIAA1111; MRXSSD; ZNF422; jumonji C domain-containing histone demethylase 1F

Product Properties

Kit components • Mouse Histone lysine demethylase PHF8 microplate
• Mouse Histone lysine demethylase PHF8 standard
• Mouse Histone lysine demethylase PHF8 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Histone lysine demethylase PHF8 (PHF8) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PHF8. No significant cross-reactivity or interference between Mouse PHF8 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PHF8 belongs to the family of ferrous iron and 2-oxoglutarate dependent oxygenases, and is active as a histone lysine demethylase with selectivity for the di-and monomethyl states. Mutations in PHF8 cause Siderius type X-linked mental retardation (XLMR). In addition to moderate mental retardation, features of the Siderius-Hamel syndrome include facial dysmorphism, cleft lip and/or cleft palate, and in some cases microcephaly. A chromosomal microdeletion on Xp11.22 encompassing all of the PHF8 and FAM120C genes and a part of the WNK3 gene was reported in two brothers with autism spectrum disorder in addition to Siderius-type XLMR and cleft lip and palate.
Gene ID 320595
Alternative PHF8; RP13-444K19.2; DKFZp686E0868; JHDM1F; KIAA1111; MRXSSD; ZNF422; jumonji C domain-containing histone demethylase 1F
Accession Q80TJ7

Image & description

Fig.1. Mouse Histone lysine demethylase PHF8 (PHF8) Standard Curve.

Fig.1. Mouse Histone lysine demethylase PHF8 (PHF8) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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