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Mouse Histone acetyltransferase MYST4 (MYST4) ELISA Kit

Mouse Histone acetyltransferase MYST4 (MYST4) ELISA Kit

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Specification

Product name Mouse Histone acetyltransferase MYST4 (MYST4) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Histone acetyltransferase MYST4 (MYST4) ELISA Kit employs a two-site sandwich ELISA to quantitate MYST4 in samples. An antibody specific for MYST4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYST4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYST4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYST4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MYST4; DKFZp313G1618; FLJ90335; KAT6B; KIAA0383; MORF; MOZ2; qkf; querkopf; MOZ-related factor; histone acetyltransferase; monocytic leukemia zinc finger protein-related factor

Product Properties

Kit components • Mouse Histone acetyltransferase MYST4 microplate
• Mouse Histone acetyltransferase MYST4 standard
• Mouse Histone acetyltransferase MYST4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Histone acetyltransferase MYST4 (MYST4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MYST4. No significant cross-reactivity or interference between Mouse MYST4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Histone acetyltransferase which may be involved in both positive and negative regulation of transcription. Required for RUNX2-dependent transcriptional activation. Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity (By similarity). Involved in cerebral cortex development.
Gene ID 54169
Alternative MYST4; DKFZp313G1618; FLJ90335; KAT6B; KIAA0383; MORF; MOZ2; qkf; querkopf; MOZ-related factor; histone acetyltransferase; monocytic leukemia zinc finger protein-related factor
Accession Q8BRB7

Image & description

Fig.1. Mouse Histone acetyltransferase MYST4 (MYST4) Standard Curve.

Fig.1. Mouse Histone acetyltransferase MYST4 (MYST4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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