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Mouse GTPase NRas (NRAS) ELISA Kit

Mouse GTPase NRas (NRAS) ELISA Kit

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Specification

Product name Mouse GTPase NRas (NRAS) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse GTPase NRas (NRAS) ELISA Kit employs a two-site sandwich ELISA to quantitate NRAS in samples. An antibody specific for NRAS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNRAS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NRAS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NRAS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative NRAS; ALPS4; N-ras; NRAS1; N-ras protein part 4; v-ras neuroblastoma RAS viral oncogene homolog

Product Properties

Kit components • Mouse GTPase NRas microplate
• Mouse GTPase NRas standard
• Mouse GTPase NRas detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse GTPase NRas (NRAS) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NRAS. No significant cross-reactivity or interference between Mouse NRAS and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).
Alternative NRAS; ALPS4; N-ras; NRAS1; N-ras protein part 4; v-ras neuroblastoma RAS viral oncogene homolog
Accession P08556

Image & description

Fig.1. Mouse GTPase NRas (NRAS) Standard Curve.

Fig.1. Mouse GTPase NRas (NRAS) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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