|Product name||Mouse GDP-fucose protein O-fucosyltransferase 2 (POFUT2) ELISA Kit|
|Applications notes||This Mouse GDP-fucose protein O-fucosyltransferase 2 (POFUT2) ELISA Kit employs a two-site sandwich ELISA to quantitate POFUT2 in samples. An antibody specific for POFUT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOFUT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POFUT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POFUT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Alternative||POFUT2; C21orf80; FUT13;|
|Kit components||• Mouse GDP-fucose protein O-fucosyltransferase 2 microplate
• Mouse GDP-fucose protein O-fucosyltransferase 2 standard
• Mouse GDP-fucose protein O-fucosyltransferase 2 detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Mouse GDP-fucose protein O-fucosyltransferase 2 (POFUT2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse POFUT2. No significant cross-reactivity or interference between Mouse POFUT2 and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||POFUT2 is an enzyme responsible for adding fucose sugars in O linkage to serine or threonine residues in Thrombospondin repeats. The protein is an inverting glycosyltransferase, which means that the enzyme uses GDP-β-L-fucose as a donor substrate and transfers the fucose in O linkage to the protein producing fucose-α-O-serine/threonine. Almost all glycosyltransferases reside in the Golgi apparatus. However, POFUT2 as well as the related enzyme POFUT1 have recently been shown to reside in the endoplasmic reticulum.The deduced protein contains 428 amino acids. RT-PCR ELISA detected moderate expression in all adult and fetal tissues and specific brain regions examined. Crystal structures of POFUT2 reveal a variation of the classical GT-B fold and identify sugar donor and TSR acceptor binding sites.|
|Alternative||POFUT2; C21orf80; FUT13;|
Fig.1. Mouse GDP-fucose protein O-fucosyltransferase 2 (POFUT2) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.