|Product name||Mouse Fibroblast growth factor 6 (FGF6) ELISA Kit|
|Applications notes||This Mouse Fibroblast growth factor 6 (FGF6) ELISA Kit employs a two-site sandwich ELISA to quantitate FGF6 in samples. An antibody specific for FGF6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFGF6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF6 bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Alternative||FGF6; HBGF-6; HST2;|
|Kit components||• Mouse Fibroblast growth factor 6 microplate
• Mouse Fibroblast growth factor 6 standard
• Mouse Fibroblast growth factor 6 detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Mouse Fibroblast growth factor 6 (FGF6) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse FGF6. No significant cross-reactivity or interference between Mouse FGF6 and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Fibroblast growth factor 6 is a protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene displayed oncogenic transforming activity when transfected into mammalian cells.The mouse homolog of this gene exhibits a restricted expression profile predominantly in the myogenic lineage, which suggested a role in muscle regeneration or differentiation.Marics et al. (1989) showed that the cloned normal FGF6 gene transformed mouse NIH 3T3 fibroblasts using both focus and tumorigenicity assays.|
|Alternative||FGF6; HBGF-6; HST2;|
Fig.1. Mouse Fibroblast growth factor 6 (FGF6) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.