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Mouse Fibroblast growth factor 4 (FGF4) ELISA Kit

Mouse Fibroblast growth factor 4 (FGF4) ELISA Kit

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Specification

Product name Mouse Fibroblast growth factor 4 (FGF4) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Fibroblast growth factor 4 (FGF4) ELISA Kit employs a two-site sandwich ELISA to quantitate FGF4 in samples. An antibody specific for FGF4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFGF4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative FGF4; HBGF-4; HST; HST-1; HSTF1; K-FGF; KFGF; fibroblast growth factor 4 splice isoform; heparin secretory transforming protein 1; human stomach cancer; transforming factor from FGF-related oncogene; kaposi

Product Properties

Kit components • Mouse Fibroblast growth factor 4 microplate
• Mouse Fibroblast growth factor 4 standard
• Mouse Fibroblast growth factor 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Fibroblast growth factor 4 (FGF4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse FGF4. No significant cross-reactivity or interference between Mouse FGF4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Fibroblast growth factor 4 is a protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its oncogenic transforming activity.This gene and FGF3, another oncogenic growth factor, are located closely on chromosome 11. Co-amplification of both genes was found in various kinds of human tumors. Studies on the mouse homolog suggested a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway.
Gene ID 14175
Alternative FGF4; HBGF-4; HST; HST-1; HSTF1; K-FGF; KFGF; fibroblast growth factor 4 splice isoform; heparin secretory transforming protein 1; human stomach cancer; transforming factor from FGF-related oncogene; kaposi
Accession P11403

Image & description

Fig.1. Mouse Fibroblast growth factor 4 (FGF4) Standard Curve.

Fig.1. Mouse Fibroblast growth factor 4 (FGF4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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