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Mouse Fibroblast Growth Factor 15 (FGF15) ELISA Kit

Mouse Fibroblast Growth Factor 15 (FGF15) ELISA Kit

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Specification

Product name Mouse Fibroblast Growth Factor 15 (FGF15) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Fibroblast Growth Factor 15 (FGF15) ELISA Kit employs a two-site sandwich ELISA to quantitate FGF15 in samples. An antibody specific for FGF15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFGF15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF15 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative FGF15

Product Properties

Kit components • Mouse Fibroblast Growth Factor 15 microplate
• Mouse Fibroblast Growth Factor 15 standard
• Mouse Fibroblast Growth Factor 15 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Fibroblast Growth Factor 15 (FGF15) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse FGF15. No significant cross-reactivity or interference between Mouse FGF15 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background FGF15 is the mouse ortholog of human FGF19 (hence there is no human FGF15).Fibroblast growth factors, or FGFs, are a family of growth factors involved in angiogenesis, wound healing, and embryonic development. The FGFs are heparin-binding proteins and interactions with cell-surface associated heparan sulfate proteoglycans have been shown to be essential for FGF signal transduction. FGFs are key players in the processes of proliferation and differentiation of wide variety of cells and tissues.Fibroblast growth factor was found in pituitary extracts by Armelin in 1973 and then was also found in a cow brain extract by Gospodarowicz et al. and tested in a bioassay which caused fibroblasts to proliferate (first published report in 1974).
Alternative FGF15

Image & description

Fig.1. Mouse Fibroblast Growth Factor 15 (FGF15) Standard Curve.

Fig.1. Mouse Fibroblast Growth Factor 15 (FGF15) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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