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Mouse FBJ murine osteosarcoma viral oncogene homolog B (FOSB) ELISA Kit

Mouse FBJ murine osteosarcoma viral oncogene homolog B (FOSB) ELISA Kit

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Specification

Product name Mouse FBJ murine osteosarcoma viral oncogene homolog B (FOSB) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse FBJ murine osteosarcoma viral oncogene homolog B (FOSB) ELISA Kit employs a two-site sandwich ELISA to quantitate FOSB in samples. An antibody specific for FOSB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFOSB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FOSB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FOSB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative FOSB; AP-1; DKFZp686C0818; G0S3; GOS3; GOSB; MGC42291; activator protein 1; oncogene FOS-B

Product Properties

Kit components • Mouse FBJ murine osteosarcoma viral oncogene homolog B microplate
• Mouse FBJ murine osteosarcoma viral oncogene homolog B standard
• Mouse FBJ murine osteosarcoma viral oncogene homolog B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse FBJ murine osteosarcoma viral oncogene homolog B (FOSB) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse FOSB. No significant cross-reactivity or interference between Mouse FOSB and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Delta FosB is a truncated splice variant of FosB. Delta FosB has been implicated in the development of drug addiction and control of the reward system in the brain, and is linked to changes in a number of other gene products such as CREB and sirtuins. "Delta FosB" levels have been found to increase upon the use of cocaine. Each subsequent dose of cocaine will continue to increase the levels of Delta FosB with no ceiling of tolerance. Increasing the levels of Delta FosB has led to increases in "Brain-Derived Neurotrophic Factor" (BDNF) levels, which in turn will increase the number of dendritic
Alternative FOSB; AP-1; DKFZp686C0818; G0S3; GOS3; GOSB; MGC42291; activator protein 1; oncogene FOS-B

Image & description

Fig.1. Mouse FBJ murine osteosarcoma viral oncogene homolog B (FOSB) Standard Curve.

Fig.1. Mouse FBJ murine osteosarcoma viral oncogene homolog B (FOSB) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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