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Mouse Fatty acid-binding protein, brain (FABP7) ELISA Kit

Mouse Fatty acid-binding protein, brain (FABP7) ELISA Kit

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Specification

Product name Mouse Fatty acid-binding protein, brain (FABP7) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Fatty acid-binding protein, brain (FABP7) ELISA Kit employs a two-site sandwich ELISA to quantitate FABP7 in samples. An antibody specific for FABP7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFABP7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FABP7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FABP7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative FABP7; B-FABP; BLBP; DKFZp547J2313; FABPB; MRG; brain lipid binding protein; mammary-derived growth inhibitor-related

Product Properties

Kit components • Mouse Fatty acid-binding protein, brain microplate
• Mouse Fatty acid-binding protein, brain standard
• Mouse Fatty acid-binding protein, brain detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Fatty acid-binding protein, brain (FABP7) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse FABP7. No significant cross-reactivity or interference between Mouse FABP7 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Fatty acid binding protein 7, brain (FABP7; also brain lipid binding protein, BLBP), is a brain fatty acid binding protein. Fatty acid binding proteins (FABPs) are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs are thought to play roles in fatty acid uptake, transport, and metabolism.FABP7 is expressed, during development, in radial glia by the activation of Notch receptors. Reelin was shown to induce FABP7 expression in neural progenitor cells via Notch-1 activation. According to one study, FABP7 binds DHA with the highest affinity among all of the FABPs.FABP7 maps onto human chromosome 6q22.31, a schizophrenia linkage region corroborated by a meta-analysis..
Gene ID 777787
Alternative FABP7; B-FABP; BLBP; DKFZp547J2313; FABPB; MRG; brain lipid binding protein; mammary-derived growth inhibitor-related
Accession Q09139

Image & description

Fig.1. Mouse Fatty acid-binding protein, brain (FABP7) Standard Curve.

Fig.1. Mouse Fatty acid-binding protein, brain (FABP7) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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