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Mouse Epidermal growth factor-like protein 7 (EGFL7) ELISA Kit

Mouse Epidermal growth factor-like protein 7 (EGFL7) ELISA Kit

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Specification

Product name Mouse Epidermal growth factor-like protein 7 (EGFL7) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Epidermal growth factor-like protein 7 (EGFL7) ELISA Kit employs a two-site sandwich ELISA to quantitate EGFL7 in samples. An antibody specific for EGFL7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEGFL7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGFL7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGFL7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative EGFL7; MGC111117; RP11-251M1.2; VE-STATIN; ZNEU1; NEU1 protein; OTTHUMP00000022597

Product Properties

Kit components • Mouse Epidermal growth factor-like protein 7 microplate
• Mouse Epidermal growth factor-like protein 7 standard
• Mouse Epidermal growth factor-like protein 7 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Epidermal growth factor-like protein 7 (EGFL7) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse EGFL7. No significant cross-reactivity or interference between Mouse EGFL7 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The deduced 275-amino acid protein has a calculated molecular mass of 29.8 kD. The mouse and human EGFL7 proteins both contain an N-terminal cleavable signal peptide followed by 2 epidermal growth factor (EGF)-like domains, and they share 78% amino acid identity. Northern blot analysis detected a transcript of about 1.6 kb only in mouse heart, lung, and kidney. In situ hybridization showed mouse Egfl7 expressed at embryonic day 7.5 exclusively in the primitive blood islands where the first endothelial cells differentiate, and later in endothelial cells of a wide range of tissues. Fluorescence-labeled Egfl7 was expressed in the endoplasmic reticulum of transfected mouse fibroblasts, and it was secreted into the culture medium.
Gene ID 353156
Alternative EGFL7; MGC111117; RP11-251M1.2; VE-STATIN; ZNEU1; NEU1 protein; OTTHUMP00000022597
Accession Q9QXT5

Image & description

Fig.1. Mouse Epidermal growth factor-like protein 7 (EGFL7) Standard Curve.

Fig.1. Mouse Epidermal growth factor-like protein 7 (EGFL7) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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