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Mouse Ephrin-B2 (EFNB2) ELISA Kit

Mouse Ephrin-B2 (EFNB2) ELISA Kit

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Specification

Product name Mouse Ephrin-B2 (EFNB2) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Ephrin-B2 (EFNB2) ELISA Kit employs a two-site sandwich ELISA to quantitate EFNB2 in samples. An antibody specific for EFNB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEFNB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EFNB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EFNB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative EFNB2; RP11-272L14.1; EPLG5; HTKL; Htk-L; LERK5; MGC126226; MGC126227; MGC126228; HTK ligand; eph-related receptor tyrosine kinase ligand 5; ephrin B2; ligand of eph-related kinase 5

Product Properties

Kit components • Mouse Ephrin-B2 microplate
• Mouse Ephrin-B2 standard
• Mouse Ephrin-B2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Ephrin-B2 (EFNB2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse EFNB2. No significant cross-reactivity or interference between Mouse EFNB2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Ephrin-B2 is a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors.Binds to the receptor tyrosine kinases EPHB4 and EPHA3. May play a role in constraining the orientation of longitudinally projecting axons.
Gene ID 13642
Alternative EFNB2; RP11-272L14.1; EPLG5; HTKL; Htk-L; LERK5; MGC126226; MGC126227; MGC126228; HTK ligand; eph-related receptor tyrosine kinase ligand 5; ephrin B2; ligand of eph-related kinase 5
Accession P52800

Image & description

Fig.1. Mouse Ephrin-B2 (EFNB2) Standard Curve.

Fig.1. Mouse Ephrin-B2 (EFNB2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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