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Mouse Eotaxin 3 (CCL26) ELISA Kit

Mouse Eotaxin 3 (CCL26) ELISA Kit

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Specification

Product name Mouse Eotaxin 3 (CCL26) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Eotaxin 3 (CCL26) ELISA Kit employs a two-site sandwich ELISA to quantitate CCL26 in samples. An antibody specific for CCL26 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCCL26 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL26 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL26 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative CCL26; MIP4A; SCYA26; IMAC; MIP4-a; TSC-1; Eotaxin 3; Chemokine(C-C-Motif)ligand 26; Thymic Stroma Chemokine-1; Small Inducible Cytokine Subfamily A Cys-Cys)Member 26

Product Properties

Kit components • Mouse Eotaxin 3 microplate
• Mouse Eotaxin 3 standard
• Mouse Eotaxin 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Eotaxin 3 (CCL26) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse CCL26. No significant cross-reactivity or interference between Mouse CCL26 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Chemokine (C-C motif) ligand 26 (CCL26) is a small cytokine belonging to the CC chemokine family that is also called Eotaxin-3, Macrophage inflammatory protein 4-alpha (MIP-4-alpha), Thymic stroma chemokine-1 (TSC-1), and IMAC. It is expressed by several tissues including heart, lung and ovary, and in endothelial cells that have been stimulated with the cytokine interleukin 4.CCL26 is chemotactic for eosinophils and basophils and elicits its effects by binding to the cell surface chemokine receptor CCR3. This gene for chemokine is located on human chromosome 7.
Alternative CCL26; MIP4A; SCYA26; IMAC; MIP4-a; TSC-1; Eotaxin 3; Chemokine(C-C-Motif)ligand 26; Thymic Stroma Chemokine-1; Small Inducible Cytokine Subfamily A Cys-Cys)Member 26

Image & description

Fig.1. Mouse Eotaxin 3 (CCL26) Standard Curve.

Fig.1. Mouse Eotaxin 3 (CCL26) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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