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Mouse Endocrine gland vascular endothelial growth factor (EG-VEGF) ELISA Kit

Mouse Endocrine gland vascular endothelial growth factor (EG-VEGF) ELISA Kit

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Specification

Product name Mouse Endocrine gland vascular endothelial growth factor (EG-VEGF) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Endocrine gland vascular endothelial growth factor (EG-VEGF) ELISA Kit employs a two-site sandwich ELISA to quantitate PROK1 in samples. An antibody specific for PROK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPROK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PROK1; EGVEGF; PK1; PRK1; black mamba toxin-related protein; mambakine

Product Properties

Kit components • Mouse Endocrine gland vascular endothelial growth factor microplate
• Mouse Endocrine gland vascular endothelial growth factor standard
• Mouse Endocrine gland vascular endothelial growth factor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Endocrine gland vascular endothelial growth factor (EG-VEGF) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PROK1. No significant cross-reactivity or interference between Mouse PROK1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The prokineticin 1 encoded by Prok1 induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner.
Gene ID 246691
Alternative PROK1; EGVEGF; PK1; PRK1; black mamba toxin-related protein; mambakine
Accession Q14A28

Image & description

Fig.1. Mouse Endocrine gland vascular endothelial growth factor (EG-VEGF) Standard Curve.

Fig.1. Mouse Endocrine gland vascular endothelial growth factor (EG-VEGF) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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