|Product name||Mouse Doublesex- and mab-3-related transcription factor C2 (DMRTC2) ELISA Kit|
|Applications notes||This Mouse Doublesex- and mab-3-related transcription factor C2 (DMRTC2) ELISA Kit employs a two-site sandwich ELISA to quantitate DMRTC2 in samples. An antibody specific for DMRTC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMRTC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMRTC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRTC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Mouse Doublesex- and mab-3-related transcription factor C2 microplate
• Mouse Doublesex- and mab-3-related transcription factor C2 standard
• Mouse Doublesex- and mab-3-related transcription factor C2 detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Mouse Doublesex- and mab-3-related transcription factor C2 (DMRTC2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DMRTC2. No significant cross-reactivity or interference between Mouse DMRTC2 and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||DMRTC2 belongs to the DMRT family. Contains 1 DM DNA-binding domain. Expressed in testis and pancreas. May be involved in sexual development. Recent advances in the evolutionary genetics of sex determination indicate that DMRT1 may be a vertebrate equivalent of the Drosophila melanogaster master sex regulator gene, doublesex. The role of DMRT1 seems to be confined to some aspects of male sex differentiation, whereas in Drosophila, doublesex has wider developmental effects in both sexes. This suggests other homologs of doublesex may exist in the vertebrate genome and encode sex-specific functions not displayed by DMRT1. Human DM genes map to three well-defined regions of chromosomes 1, 9, and 19 (one gene on chromosome 19 having an additional homolog on chromosome X).|
Fig.1. Mouse Doublesex- and mab-3-related transcription factor C2 (DMRTC2) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.