Login Register
English
0

Cart

$ 0

Mouse Doublesex- and mab-3-related transcription factor A2 (DMRTA2) ELISA Kit

Mouse Doublesex- and mab-3-related transcription factor A2 (DMRTA2) ELISA Kit

Views(113) Publications(0) Catalog no(KTE71239)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Mouse Doublesex- and mab-3-related transcription factor A2 (DMRTA2) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Doublesex- and mab-3-related transcription factor A2 (DMRTA2) ELISA Kit employs a two-site sandwich ELISA to quantitate DMRTA2 in samples. An antibody specific for DMRTA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMRTA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMRTA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRTA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DMRTA2; OTTHUMP00000211162

Product Properties

Kit components • Mouse Doublesex- and mab-3-related transcription factor A2 microplate
• Mouse Doublesex- and mab-3-related transcription factor A2 standard
• Mouse Doublesex- and mab-3-related transcription factor A2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Doublesex- and mab-3-related transcription factor A2 (DMRTA2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DMRTA2. No significant cross-reactivity or interference between Mouse DMRTA2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background DMRTA2 is involved in gonadal differentiation. The gene is part of five novel human DM genes that map to three well-defined regions of chromosomes 1, 9, and 19. Several studies have reported QTL in various species and strains of tilapia, regions contributing to sex determination have been identified on linkage groups 1, 3, and 23. Genes contributing to sex-specific mortality have been detected on linkage groups 2, 6, and 23. Mapping of Dax1 joined LG16 and LG21 into a single linkage group. The Amh and Dmrta2 genes were mapped to two distinct regions of LG23. The Amh gene was mapped 5 cM from UNH879 within a QTL region for sex determination and 2 cM from UNH216 within a QTL region for sex-specific mortality. Dmrta2 was mapped 4 cM from UNH848 within another QTL region for sex determination.
Gene ID 242620
Alternative DMRTA2; OTTHUMP00000211162
Accession A2A9A2

Image & description

Fig.1. Mouse Doublesex- and mab-3-related transcription factor A2 (DMRTA2) Standard Curve.

Fig.1. Mouse Doublesex- and mab-3-related transcription factor A2 (DMRTA2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Mouse Doublesex- and mab-3-related transcription factor A2 (DMRTA2) ELISA Kit”