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Mouse Doublesex- and mab-3-related transcription factor 3 (DMRT3) ELISA Kit

Mouse Doublesex- and mab-3-related transcription factor 3 (DMRT3) ELISA Kit

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Specification

Product name Mouse Doublesex- and mab-3-related transcription factor 3 (DMRT3) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Doublesex- and mab-3-related transcription factor 3 (DMRT3) ELISA Kit employs a two-site sandwich ELISA to quantitate DMRT3 in samples. An antibody specific for DMRT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMRT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMRT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DMRT3; DMRTA3; MGC142144; DMRT-like family A3; testis-specific protein

Product Properties

Kit components • Mouse Doublesex- and mab-3-related transcription factor 3 microplate
• Mouse Doublesex- and mab-3-related transcription factor 3 standard
• Mouse Doublesex- and mab-3-related transcription factor 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Doublesex- and mab-3-related transcription factor 3 (DMRT3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DMRT3. No significant cross-reactivity or interference between Mouse DMRT3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Vertebrate DM domain genes encode a novel group of proteins related to the Drosophila doublesex and Caenorhabditis elegans mab-3 transcription factors. It is shown here that the recently identified gene, DMRT3, has a restricted embryonic expression profile that is conserved in chicken and mouse embryos. DMRT3 is expressed primarily in the forebrain, neural tube and nasal placode of both species. In the chicken, DMRT3 is also expressed in newly forming tail somites at early developmental stages and, later, in the Müllerian ducts of the urogenital system.The sequence of this gene is defined by 14 GenBank accessions from 13 cDNA clones, some from colon, PCR rescued clones (seen 2 times), thalamus (2), colon (once), colon tumor RER+ (once), lung (once), parathyroid gland (once), parathyroid tumor (once) and 4 other tissues.
Gene ID 240590
Alternative DMRT3; DMRTA3; MGC142144; DMRT-like family A3; testis-specific protein
Accession Q80WT2

Image & description

Fig.1. Mouse Doublesex- and mab-3-related transcription factor 3 (DMRT3) Standard Curve.

Fig.1. Mouse Doublesex- and mab-3-related transcription factor 3 (DMRT3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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