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Mouse DNA polymerase nu (POLN) ELISA Kit

Mouse DNA polymerase nu (POLN) ELISA Kit

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Specification

Product name Mouse DNA polymerase nu (POLN) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse DNA polymerase nu (POLN) ELISA Kit employs a two-site sandwich ELISA to quantitate POLN in samples. An antibody specific for POLN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative POLN; RP11-317B7.1; POL4P; DNA polymerase N; DNA polymerase POL4P; DNA-directed DNA polymerase nu

Product Properties

Kit components • Mouse DNA polymerase nu microplate
• Mouse DNA polymerase nu standard
• Mouse DNA polymerase nu detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse DNA polymerase nu (POLN) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse POLN. No significant cross-reactivity or interference between Mouse POLN and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background POLN is predicted to contain a fingers-thumb-palm structure conserved in the PolA family. Northern blot analysis of human tissues detected an approximately 4-kb transcript in testis, heart, and skeletal muscle, and a 2-kb transcript in heart, liver, skeletal muscle, kidney, and pancreas. The 2-kb transcript was detected in a panel of human cancer cell lines, and the 4-kb transcript was detected only in a chronic myelogenous leukemia cell line. Both POLN and DNA helicase HEL308 showed high expression in human and mouse testis. In situ hybridization of mouse testis tissue localized the highest Poln expression to meiotic spermatocytes and postmeiotic round spermatids, while Hel308 expression occurred in primary spermatocytes but was not detected in round spermatids.
Gene ID 272158
Alternative POLN; RP11-317B7.1; POL4P; DNA polymerase N; DNA polymerase POL4P; DNA-directed DNA polymerase nu
Accession Q7TQ07

Image & description

Fig.1. Mouse DNA polymerase nu (POLN) Standard Curve.

Fig.1. Mouse DNA polymerase nu (POLN) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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