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Mouse DmX-like protein 1 (DMXL1) ELISA Kit

Mouse DmX-like protein 1 (DMXL1) ELISA Kit

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Product name Mouse DmX-like protein 1 (DMXL1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse DmX-like protein 1 (DMXL1) ELISA Kit employs a two-site sandwich ELISA to quantitate DMXL1 in samples. An antibody specific for DMXL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMXL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMXL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMXL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DMXL1; DKFZp779O1239; FLJ44772;

Product Properties

Kit components • Mouse DmX-like protein 1 microplate
• Mouse DmX-like protein 1 standard
• Mouse DmX-like protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse DmX-like protein 1 (DMXL1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DMXL1. No significant cross-reactivity or interference between Mouse DMXL1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background By EST database searching for sequences showing homology to Drosophila DmX and by use of primers designed for RT-PCR from human placental mRNA, Kraemer et al. (2000) cloned a nearly full-length homolog, which they designated DMX-like-1. The DMXL1 cDNA encodes a deduced 3,027-amino acid protein with a calculated mass of approximately 338 kD. The protein belongs to the superfamily of WD repeat proteins, most of which have regulatory functions, and contains at least 28 WD repeat units. DMXL1 shows a high level of evolutionary conservation. By searching EST databases with the complete cDNA sequence as query, Kraemer et al. (2000) found that DMXL1 is expressed in a variety of tissues, including bone, breast, eye, foreskin, heart, parathyroid, small intestine, testis, tonsils, uterus, and whole embryo.
Gene ID 240283
Alternative DMXL1; DKFZp779O1239; FLJ44772;
Accession Q6PNC0

Image & description

Fig.1. Mouse DmX-like protein 1 (DMXL1) Standard Curve.

Fig.1. Mouse DmX-like protein 1 (DMXL1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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