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Mouse Disrupted in schizophrenia 1 protein (DISC1) ELISA Kit

Mouse Disrupted in schizophrenia 1 protein (DISC1) ELISA Kit

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Specification

Product name Mouse Disrupted in schizophrenia 1 protein (DISC1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Disrupted in schizophrenia 1 protein (DISC1) ELISA Kit employs a two-site sandwich ELISA to quantitate DISC1 in samples. An antibody specific for DISC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDISC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DISC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DISC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DISC1; RP4-730B13.1; C1orf136; FLJ13381; FLJ21640; FLJ25311; FLJ41105; KIAA0457; SCZD9;

Product Properties

Kit components • Mouse Disrupted in schizophrenia 1 protein microplate
• Mouse Disrupted in schizophrenia 1 protein standard
• Mouse Disrupted in schizophrenia 1 protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Disrupted in schizophrenia 1 protein (DISC1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DISC1. No significant cross-reactivity or interference between Mouse DISC1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Disrupted-in-Schizophrenia 1 or DISC1 is a protein with a wide array of functions. It participates in cell growth, movement, axonal growth and transport, neural positioning. Disruptions in DISC1 structure may play an important role at least in some cases of schizophrenia, however the evidence from genetic linkage and association studies suggests that variants of DISC1 do not play a significant role in most cases of schizophrenia, although DISC1 may be more important in bipolar disorder. In humans, this protein is encoded by the DISC1 gene. This gene encodes a protein with multiple coiled coil motifs which is located in the nucleus, cytoplasm and mitochondria. The protein is involved in neurite outgrowth and cortical development through its interaction with other proteins.
Gene ID 244667
Alternative DISC1; RP4-730B13.1; C1orf136; FLJ13381; FLJ21640; FLJ25311; FLJ41105; KIAA0457; SCZD9;
Accession Q811T9

Image & description

Fig.1. Mouse Disrupted in schizophrenia 1 protein (DISC1) Standard Curve.

Fig.1. Mouse Disrupted in schizophrenia 1 protein (DISC1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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