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Mouse Dipeptidyl peptidase 9 (DPP9) ELISA Kit

Mouse Dipeptidyl peptidase 9 (DPP9) ELISA Kit

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Specification

Product name Mouse Dipeptidyl peptidase 9 (DPP9) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Dipeptidyl peptidase 9 (DPP9) ELISA Kit employs a two-site sandwich ELISA to quantitate DPP9 in samples. An antibody specific for DPP9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDPP9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPP9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPP9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DPP9; DKFZp762F117; DPRP2; FLJ16073; dipeptidyl peptidase IV-related protein-2; dipeptidylpeptidase 9

Product Properties

Kit components • Mouse Dipeptidyl peptidase 9 microplate
• Mouse Dipeptidyl peptidase 9 standard
• Mouse Dipeptidyl peptidase 9 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Dipeptidyl peptidase 9 (DPP9) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DPP9. No significant cross-reactivity or interference between Mouse DPP9 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Dipeptidyl peptidase 9 is a member of the S9B family in clan SC of the serine proteases. The protein has been shown to have post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Although the activity of this protein is similar to that of dipeptidyl peptidase 4 (DPP4), it does not appear to be membrane bound. In general, dipeptidyl peptidases appear to be involved in the regulation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer. Several transcript variants of this gene have been described but not fully characterized.
Gene ID 224897
Alternative DPP9; DKFZp762F117; DPRP2; FLJ16073; dipeptidyl peptidase IV-related protein-2; dipeptidylpeptidase 9
Accession Q8BVG4

Image & description

Fig.1. Mouse Dipeptidyl peptidase 9 (DPP9) Standard Curve.

Fig.1. Mouse Dipeptidyl peptidase 9 (DPP9) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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