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Mouse Dickkopf-related protein 2 (DKK2) ELISA Kit

Mouse Dickkopf-related protein 2 (DKK2) ELISA Kit

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Specification

Product name Mouse Dickkopf-related protein 2 (DKK2) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Dickkopf-related protein 2 (DKK2) ELISA Kit employs a two-site sandwich ELISA to quantitate DKK2 in samples. An antibody specific for DKK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDKK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DKK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DKK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DKK2; DKK-2; dickkopf 2; dickkopf homolog 2; dickkopf related protein-2

Product Properties

Kit components • Mouse Dickkopf-related protein 2 microplate
• Mouse Dickkopf-related protein 2 standard
• Mouse Dickkopf-related protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Dickkopf-related protein 2 (DKK2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DKK2. No significant cross-reactivity or interference between Mouse DKK2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Dickkopf-related protein 2 is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6).Northern blot analysis revealed expression of 4.0- and 4.5-kb DKK2 transcripts in heart, brain, skeletal muscle, and lung. Western blot analysis showed that DKK2 is secreted as a 15- to 17-kD protein. Functional analysis determined that DKK2 does not block Xenopus Wnt8 induction of a secondary axis in frog embryos.
Gene ID 56811
Alternative DKK2; DKK-2; dickkopf 2; dickkopf homolog 2; dickkopf related protein-2
Accession Q9QYZ8

Image & description

Fig.1. Mouse Dickkopf-related protein 2 (DKK2) Standard Curve.

Fig.1. Mouse Dickkopf-related protein 2 (DKK2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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