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Mouse DEP domain-containing protein 5 (DEPDC5) ELISA Kit

Mouse DEP domain-containing protein 5 (DEPDC5) ELISA Kit

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Specification

Product name Mouse DEP domain-containing protein 5 (DEPDC5) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse DEP domain-containing protein 5 (DEPDC5) ELISA Kit employs a two-site sandwich ELISA to quantitate DEPDC5 in samples. An antibody specific for DEPDC5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDEPDC5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEPDC5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEPDC5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DEPDC5; LL22NC03-113A11.1; DEP.5; DKFZp779P2027; OTTHUMP00000028946

Product Properties

Kit components • Mouse DEP domain-containing protein 5 microplate
• Mouse DEP domain-containing protein 5 standard
• Mouse DEP domain-containing protein 5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse DEP domain-containing protein 5 (DEPDC5) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DEPDC5. No significant cross-reactivity or interference between Mouse DEPDC5 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background DEPDC5 encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by DEPDC5 is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in DEPDC5 are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of DEPDC5 has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants.
Gene ID 277854
Alternative DEPDC5; LL22NC03-113A11.1; DEP.5; DKFZp779P2027; OTTHUMP00000028946
Accession P61460

Image & description

Fig.1. Mouse DEP domain-containing protein 5 (DEPDC5) Standard Curve.

Fig.1. Mouse DEP domain-containing protein 5 (DEPDC5) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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