Login Register
English
0

Cart

$ 0

Mouse Deoxyguanosine kinase, mitochondrial (DGUOK) ELISA Kit

Mouse Deoxyguanosine kinase, mitochondrial (DGUOK) ELISA Kit

Views(156) Publications(0) Catalog no(KTE71298)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Mouse Deoxyguanosine kinase, mitochondrial (DGUOK) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Deoxyguanosine kinase, mitochondrial (DGUOK) ELISA Kit employs a two-site sandwich ELISA to quantitate DGUOK in samples. An antibody specific for DGUOK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDGUOK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DGUOK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DGUOK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DGUOK; dGK; deoxyguanosine kinase; mitochondrial

Product Properties

Kit components • Mouse Deoxyguanosine kinase, mitochondrial microplate
• Mouse Deoxyguanosine kinase, mitochondrial standard
• Mouse Deoxyguanosine kinase, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Deoxyguanosine kinase, mitochondrial (DGUOK) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DGUOK. No significant cross-reactivity or interference between Mouse DGUOK and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background In mammalian cells, the phosphorylation of purine deoxyribonucleosides is mediated predominantly by two deoxyribonucleoside kinases, cytosolic deoxycytidine kinase and mitochondrial deoxyguanosine kinase. The protein encoded by DGUOK is responsible for phosphorylation of purine deoxyribonucleosides in the mitochondrial matrix. In addition, this protein phosphorylates several purine deoxyribonucleoside analogs used in the treatment of lymphoproliferative disorders, and this phosphorylation is critical for the effectiveness of the analogs. Alternative splice variants encoding different protein isoforms have been described for DGUOK.
Gene ID 27369
Alternative DGUOK; dGK; deoxyguanosine kinase; mitochondrial
Accession Q9QX60

Image & description

Fig.1. Mouse Deoxyguanosine kinase, mitochondrial (DGUOK) Standard Curve.

Fig.1. Mouse Deoxyguanosine kinase, mitochondrial (DGUOK) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Mouse Deoxyguanosine kinase, mitochondrial (DGUOK) ELISA Kit”